special edition: rapid pathogen detection

PathoGenetix to launch low cost model of Resolution system at IAFP

The first generation of the Resolution system

PathoGenetix will introduce a low cost model of its pathogen detection machine at the International Association for Food Protection (IAFP) meeting next week.

The firm said the Resolution microbial genotyping system will be presented at the event July 31 - August 3 in Missouri. It can identify pathogens in hours from mix cultures. 

The Resolution system performs molecular serotyping of pathogens from a presumptive positive enriched food sample with no isolate required.

Specificity is comparable to Pulsed Field Gel Electrophoresis (PFGE).

Reduced instrument cost

Hamid Shirkhan, CEO of PathoGenetix, said with the new design, the price of the instrument and consumables have been reduced by 50%.

“The system automatically extracts, purifies and sequence-specific labels genomic DNA with fluorescent probes,” he told FoodQualityNews.

“Proprietary Genome Sequence Scanning (GSS) technology measures barcodes of microbial DNA as it flows through a microfluidic chip at 10 million base pairs per second.

“These genomic barcodes are compared to a barcode database resulting in molecular serotype/straintype identification.”  

Serotyping assays are currently available for Salmonella and shiga toxin-producing E. coli (STEC). 

Since initial production release in 2015, PathoGenetix has been developing the new generation model of Resolution and reducing cost of the instrument.

The firm also has a collaboration agreement with FDA and CDC to test the system for different strains of bacteria and is in the process of applying for AOAC validation.

Toxic Reports acquired the assets of PathoGenetix, including all intellectual property, designs and prototype instruments at the start of 2015.

Shirkhan said the system is positioned in the laboratory workflow as a confirmatory test of a presumptive positive food sample.

“LoD is 1% of a total 500 ng load. Resolution performs preparation of eight samples in parallel followed by a one-sample-at-a-time detection and analysis. Time to first result is 4.5 hours. All analysis is completed in eight hours,” he said.

“We have reduced the cost of manufacturing which enables us to reduce the end user cost from $250,000 to $97,000.

“The cost reduction also enables us to offer a reagent rental program which eliminates the large capital purchase barrier for customers by amortizing the instrument cost through higher reagent/assay prices.”

The fully automated sample preparation and detection enables the firm to offer what it called a ‘very competitive product’ that could replace PGFE. 

“The speed and simplicity combined with low cost consumables makes the Resolution microbial system an ideal tool for routine analysis,” said Shirkan.  

“With the Resolution product, food laboratories can run the system automatically and unattended to identify pathogens from a mixed culture in less than five hours at the consumable cost of less than $68. The system process eight samples simultaneously.”

Shirkhan described the future as looking ‘very promising’.

“The need for simpler, faster and lower cost bacteria identification system and consumables makes our bacteria genotyping system an ideal tool for bacteria identification,” he said.

“This spans multiple markets such as food safety, microbiome monitoring as well as rapid bacteria identification for critical care.”

Related News

The Crystal Diagnostics (CDx) Xpress System

CDx system backed to screen Listeria on environmental surfaces

© iStock/nikesidoroff

Parameters to assess performance of dPCR assays

Picture: F Cubed. The NESDEP IU test kit

F Cubed pushes ahead with launch of pathogen detection tech

Sample6 and Sprout Creek Farm case study

‘My biggest food safety challenge is effectively managing my environment’

© iStock/arinarici

Preventing false-positive results in your pathogen food safety program


Lexagene to have prototype of on-site testing device by end of year

The Crystal Diagnostics (CDx) Xpress System

CDx system backed to screen Listeria on environmental surfaces

Picture: © iStock/Wiktory

DNA Diagnostic’s Salmonella test backed by NordVal

© iStock/nikesidoroff

Parameters to assess performance of dPCR assays

© iStock/arinarici

Preventing false-positive results in your pathogen food safety program

Toxic Reports acquires assets of PathoGenetix

PathoGenetix assets acquired ahead of commercialisation

Resolution Microbial Genotyping System

PathoGenetix begins production of pathogen detection tech

Related Products

See more related products

Submit a comment

Your comment has been saved

Post a comment

Please note that any information that you supply is protected by our Privacy and Cookie Policy. Access to all documents and request for further information are available to all users at no costs, In order to provide you with this free service, William Reed Business Media SAS does share your information with companies that have content on this site. When you access a document or request further information from this site, your information maybe shared with the owners of that document or information.